Three days course for those embryologists who need to implement the trophectoderm biopsy (or the cleavage stage biopsy) in their routine. Also interesting for those embryologists who are already performing biopsies but would like to have an upgrade to learn new tips and tricks on all the aspects related with the blastocyst biospy.

For further information please contact us at training@embryotools.com

SKU: TRN20-IN-005 Categories: ,


Embryo biopsy: How to implement a successful PGT program


Aims and Goals of the Course:

To understand fundamental principles of blastocyst culture, biopsy, genetic diagnosis, and cryopreservation techniques. To have an overview of current experiences and outcomes and acquire experience on how to setup and manage a successful Day-5 culture program. To have intensive hands-on in blastocysts biopsy (and cleavage stage if needed) applying the “pulling” and the “flicking” method on an unlimited number of blastocyst at different expansion stages. To practice cryopreservation after biopsy, laser assisted hatching, tubing of cells for genetic analysis and validation of the number of cells biopsied.

Scientific Program:

Theoretical Sessions (30-45 min duration):

  • “Embryo Biopsy -techniques and suggestions”

Hands-On Sessions:

  • Preparation of biopsy and embryo culture dishes.
  • Setting up of the microscope and the laser.
  • Biopsy of early, expanded and fully hatched blastocysts using the “pulling” and the “flicking” techniques with unlimited samples and live demonstration for each case.
  • Preparation of material for the tubing of the biopsied cells genetic analysis.
  • Cryopreservation, thawing and culture of blastocysts after biopsy.
  • Laser AH in thawed blastocysts.
  • Validation of the number of cells biopsied under an epifluorescence microscope.
  • Video recording and photos of the procedures performed.

Team of embryologists providing the training:
Gloria Calderón, PhD; Nuno Costa-Borges, PhD; Enric Mestres, PhD;  Mònica Acacio, MSc; Carolina Castelló, BSc.

Certificate of attendance will be awarded to participants completing the workshop

Dates: Three days (from 09:00h to 16:30h)

Prices: 3600 Euros

Depending on your experience and needs we can modify the training course by adding extra days to the course.

Embryotools’ Laboratories
Avenida Doctor Marañón, 8
Parc Científic de Barcelona
08028 Barcelona, Spain

General information

Workshop location

Embryotools S.L.

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Embryotools S.L. 41.381236, 2.116885

We are next to the Barcelona Football Stadium

Parc Científic de Barcelona
Av. Doctor Marañon 8
08028 Barcelona (Spain)
Phone: + 34 934 497 198


There are several hotel options to stay in Barcelona.

To help you on finding the best option for you, here you have a list of hotels near our facilities.

The NH hotels and Madanis hotel offers a special discount to our trainees. Ask for it!

Hotel Madanis Barcelona

Description 4 stars hotel GL
Address  Riera Blanca, 10 – 08903 – Barcelona
Distance to Embryotools 10 min walking
Information and booking  https://www.hotelmadanis.com/
Phone  (+34) 934 470 006

Hotel NH Barcelona Stadium

Description 4 stars hotel GL
Address Travessera de les Corts, 150-152, 08028 Barcelona
Distance to Embryotools 10 min walking
Information and booking http://www.nh-hoteles.es/hotel/nh-barcelona-stadium
Phone (+34) 933 399 050

Hotel NH Collection Barcelona Constanza

Description 4 stars hotel
Address Avenida Diagonal 555

08028 Barcelona

Distance to Embryotools 15 min in tram or 25 min walking
Information and booking http://www.nh-hotels.com/hotel/nh-collection-barcelona-constanza
Phone (+34) 932 811 500

Hotel Catalonia Rigoletto

Description 5 stars hotel
Address Carrer Sabino de Arana, 22 – 24,

08028 Barcelona

Distance to Embryotools 12 min walking
Information and booking http://www.hoteles-catalonia.com


Barcelona is a friendly city for tourists easy to go around. You can find information on how to move around in these two web sites:



The closest public transport stops to arrive to Embryotools are the following:


  • V1 Mini Estadi – FC Barcelona
  • H8 Les Corts- Arizala
  • 113 Mini Estadi Palau Blaugrana
  • 101 Fútbol Club Barcelona
  • 113, 33, 67, 7, 75, H6 Palau Reial


  • Green Line- L3- Palau Reial
  • Blue Line- L5- Collblanc
  • Orange Line- L9- Zona Universitaria (from airport)


  • All lines- Av Xile


  • You can also book a taxi through Whatsapp at the number: +34 675613072.  Please specify that you are coming to an Embryotools course and the flight number information. A friendly Embryotools taxi driver will pick you up at the airport to take you to hotel or to our facilities.


Classes can be conducted in English, Spanish, French or Portuguese.

For other languages, translation services can be arranged.


Informal for all occasions. We will provide with special clothing and clogs to work inside the lab. If you need a special size, please let us know.


Some participants may require a visa in order to enter Spain.

Please check the following link in english or spanish where you can download a Welcome letter. You can fill it in and send it back to us so that we can sign it and return it to you to present it to the Spanish embassy in your country.

Lunch break

Lunch break time is flexible to the lab’s needs. The lunch is included in the price of the course, you will join the Embryotools team during the lunch break at the Scientific Park’s restaurant where a fast line menu with different options is available.