描述

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Cryopreservation (Oocytes, Embryos and Blastocysts) and Assisted Hatching

Aims and Goals of the Course:

This three-days training course is specifically designed for Embryologists interested in acquiring practical skills and updating their knowledge on oocyte, embryo, and blastocyst cryopreservation techniques. The course will focus on didactic and intensive hands-on practical lessons on the vitrification system and media that the embryologist needs to implement, pointing out the tips and tricks to achieve consistent results. Throughout the three-days, embryologists will have the opportunity to vitrify and warm unlimited number of oocytes and embryos needed to understand how important little details can affect results. The course also covers the fundamental principles of the assisted hatching procedure and its multiple applications in different developmental stages. Introduction to the Laser Assisted Hatching and familiarization with different operational modes. Evaluation and discussion about the effect of the size of the hole on the developing embryo by culturing treated embryos in a TL system.

Scientific Program:

Theoretical Sessions (30-45 min duration):

• “Oocyte and Embryo Vitrification: Theoretical and practical aspects”

Hands-On Sessions:

• Vitrification and warming of oocytes, using the vitrification system of choice.
• Vitrification and warming of cleavage stage and blastocysts mouse embryos using the vitrification system of choice.
• Tips and hinds to maximize results.
• Assisted hatching of warmed embryos.
• Culture of warmed embryos and analysis of their development.

Team of embryologists providing the training:
Nuno Costa-Borges, PhD; Carolina Castelló, BSc; Enric Mestres, PhD; Mònica Acacio, MSc; Queralt Matia; MSc.

Certificate of attendance will be awarded to participants completing the workshop

Dates: Three days from 09:00h to 16:30h (with a coffee break and 30 min lunch pause).

Prices: 3000 Euros.

Depending on your experience and needs we can modify the training course by adding extra days to the course.

Location:
Embryotools’ Laboratories
Avenida Doctor Marañón, 8
Parc Científic de Barcelona
08028 Barcelona, Spain