The continuous advances in assisted reproductive technologies have had an enormous impact on the animal breeding industry. With extensive experience in assisted reproduction, our scientific team has successfully translated research into commercial breeding programs. In 2009, we successfully cloned the first animals in Spain and in 2012, using an innovative micromanipulation technique, we were the first in Europe to obtain sex-selected horses from extra-large (XL, > 300μm) blastocysts that had been previously collapsed and vitrified. More recently, in 2020, we obtained the first foal 100% made in Spain, following the application of the ovum-pick-up (OPU), in vitro maturation and intracytoplasmic sperm injection (ICSI) techniques. Currently, the ICSI technique is an established procedure in our laboratory that is performed routinely and offered commercially in collaboration with veterinarians from around Europe with high success rates.
As a premier center for equine assisted reproduction with demonstrated experience and state of the art facilities, we offer breeders from around the world the most advanced assisted reproduction techniques to solve infertility problems or increase reproductive efficiencies in elite horses.
Intracytoplasmic sperm injection (ICSI)
Intracytoplasmic sperm injection (ICSI) is an in vitro fertilization procedure recommended for mares that have not been able to establish a pregnancy with conventional insemination techniques, or when there is interest in obtaining embryos in periods when they are competing. The technique can also be very efficient in generating embryos from limited sperm samples with high economic value, offering higher success rates than those that can be obtained with traditional insemination methods. The ICSI technique is offered commercially in partnership with veterinarians across Europe with proven experience in OPU. Once the oocytes have been extracted from the mare, they are sent to our laboratory, where they are matured in vitro and subsequently inseminated. The blastocysts resultant from the ICSI sessions can then be vitrified and later on shipped to any reproductive center to be transferred. We are flexible in scheduling the ICSI sessions, however, it is preferable to receive oocytes during the weekdays to avoid shipping and courier problems that sometimes occur on weekends. We collaborate with reference centers (e.g., http://brancaleoneteam.com) that have a large catalog of samples from the best stallions in the world with the possibility of being used in ICSI sessions.
Personalized training sessions in OPU can be arranged to veterinarians who intend to implement the OPUN technique in their services portfolio. The training is offered by internationally renowned veterinarians who have been pioneers in the development and application of the technique in the most prestigious equine breeding centers worldwide.
Embryo biopsy for genetic diagnosis
Embryo biopsy is based on the extraction of a reduced number of cells from an embryo at an early stage of development, without compromising its later development. The biopsied cells are immediately processed for genetic analysis and the results are obtained before the embryo is transferred to a recipient. Using this technique, it is possible to select the sex or color of the fur of the future animal as well as to avoid the transfer of an embryo carrying a mutation or chromosomal abnormality that causes a disease. The technique can be applied to blastocysts obtained from insemination or by ICSI.
Blastocyst collapse and vitrification
We have extensive experience in the most advanced embryo vitrification methods. Embryos can be cryopreserved at different stages of development with high success rates and stored in liquid nitrogen indefinitely until our clients decide to transfer them to recipients. In the case of large size blastocysts (> 300-1500 µm), a micromanipulation technique is applied that allows the collapse of the embryo prior to vitrification, increasing success rates.
The genetics of the most valuable horses can now be preserved to guarantee that their bloodline will not be lost and they can be cloned in the future. For this simple procedure, a small biopsy sample is taken from the chosen animal by a veterinarian and immediately shipped to our laboratory in a biopsy kit and container provided by us. For better results, we recommend the biopsy to be performed when the animal is still healthy. In cases where an animal dies suddenly, a tissue sample should be obtained immediately and kept refrigerated until our biopsy kit arrives. After that it should be sent to our laboratory immediately.
Cloning is a fascinating technology that allows the production of an animal genetically identical to an existing one. All that is required is a small biopsy sample of the animal to be cloned, as described in the genetic preservation service. Once we receive it, our scientific team will do the rest.
This technology allows our customers to greatly expand the reproductive potential of their high performance animals or to reduce the impact of an unexpected injury or death. All cloned animals are subjected to DNA testing to confirm their identities and to veterinary examinations to confirm their health status.