Description

Implementing a robust cryopreservation program: about vitrification, warming and assisted hatching

Aims and Goals of the Course:

This three-days training course is specifically designed for Embryologists interested in acquiring practical skills and updating their knowledge on oocyte, embryo, and blastocyst cryopreservation techniques. The course will focus on didactic and intensive hands-on practical lessons on the vitrification system and media that the embryologist needs to implement, pointing out the tips and tricks to achieve consistent results. Throughout the three-days, embryologists will have the opportunity to vitrify and warm unlimited number of oocytes and embryos needed to understand how important small details can affect results. The course also covers the fundamental principles of the assisted hatching procedure and its multiple applications in different developmental stages. Introduction to the Laser Assisted Hatching and familiarization with different operational modes. Evaluation and discussion about the effect of the size of the hole on the developing embryo by culturing treated embryos in a TL system. At the end of the course the trainee will have enough information to implement a robust cryopreservation program and identify the possible troubleshooting.

Scientific Program:

Theoretical Sessions (30-45 min duration):

• “Advises and recommendations on oocyte and embryo vitrification and warming” by Ivette Vanrell

Hands-On Sessions:

• Vitrification and warming of oocytes, using the Cryotop open and closed vitrification system
• Vitrification and warming of cleavage stage and blastocysts mouse embryos using Cryotop open and closed
• Tips and hinds to maximize results
• Assisted hatching of warmed embryos
• Culture of warmed embryos and analysis of their development

Team of embryologists providing the training:
Gloria Calderón, PhD; Nuno Costa-Borges, PhD; Enric Mestres, MSc; Ivette Vanrell, MSc; María García, MSc and Mònica Acacio, Msc.

Certificate of attendance will be awarded to participants completing the workshop for a total of 24 hours

Prices: 2750 Euros (+ VAT if applicable) for a three days course

Location:
Embryotools’ Laboratories
Avenida Doctor Marañón, 8
Parc Científic de Barcelona
08028 Barcelona, Spain

Sponsored by ASEBIR.